By J. Joosse, W. P. M. Geraerts (auth.), A. B. Borkovec, Edward. P. Masler (eds.)

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In addition, several amino acid substitutions occur in other peptide domains. As a result, each of the two CDCH genes encodes a different, but overlapping set of neuropeptides which are thought to control various aspects of the egg-laying action pattern. In Aplysia, the ELH gene family comprises 5 distinct genes, of which 3 have been fully characterized (Fig. 8). The peptide A and B genes are considerably smaller than the ELH gene, due to the deletion of a 240 base pair DNA segment which encodes 80 amino acids.

J. Invert. Reprod. 7,249-257. B. and Joosse J. (1989) Central and peripheral expression of genes coding for egg-laying inducing and insulin-related peptides in a snail. Arch. Histol. Cytol. 52, suppl. In press. , Nieuwendijk, The Netherlands, 1975). Vreugdenhil E. (1988) The molecular basis of egg laying and egg-laying behaviour in the freshwater snail Lymnaea stagnalis. Thesis, Vrije Universiteit, Amsterdam. , Glide G. J. ln Neurohormones in invertebrates. Seminar Series Soc. expo Bioi. C. ) pp.

3. Apparatus used by the authors for loading water-diluted fractions from RPLC onto a column for subsequent RPLC separation. A low pressure slider valve is used before the pump in the liquid chromatograph to select flow (normally from the water reservoir) to a polypropylene reservoir holding the diluted solution. d. column with this device. Isolation of Neuropeptides 49 A related, but more serious, problem is that the propensity of peptides to adsorb is aggravated greatly by concentrating the solvent.

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