By Jean-Marie Exbrayat
content material: Histological and Histochemical equipment with mild Microscopy Classical equipment of Visualization; Jean-Marie Exbrayat Histochemical tools; Jean-Marie Exbrayat Enzyme Histochemistry tools; Jeanne Estabel Visualization of mobilephone Proliferation; Francoise Giroud and Marie-Paule Montmasson Visualization of Apoptosis; Claire Brun and Elara Moudilou In Situ High-Speed Tissue Proteomic; Francoise Giroud and Marie-Paule Montmasson guidance of goods for Histology and Histochemistry; Jean-Marie Exbrayat Cytological equipment in Electron Microscopy normal suggestions in Electron Microscopy; Jean-Marie Exbrayat Cytochemical suggestions; Jean-Marie Exbrayat Ultrastructural Cytoenzymology; Chantal de Chastellier The Acetylation technique; Marc Thiry and Nicolas Thelen training of goods for Electron Microscopy; Jean-Marie Exbrayat snapshot Quantification photograph Quantification in Histology and Cytology; Yves Tourneur and Leon Espinosa Index
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The solution and fragments are then placed into a mold and hardened with formalin. 5 Double Embedding: Agar-Agar and Paraffin Agar-agar and paraffin double embedding is particularly useful for oocytes, or small eggs or also pituitary organs, which must be sectioned according to an orientated draft. 1 General Principle Agar-agar and paraffin double embedding is used to cut small tissue fragments at ambient temperature that are difficult to embed in a classic paraffin block. This embedding has all the advantages of normal paraffin embedding.
Stick the sections with gelatinous water at 1%. Eliminate celloidin oil of cloves, ethanol–ether solution, and 100% ethanol. As for water adhesion, it is necessary to use perfectly cleaned slides. For that, slides are washed with ethanol and hydrochloric acid (1 vol/1 vol). Then they must be rinsed with distilled water, or purchase washed and oil-free slides. Sections are ready to be stained. 3 After Staining • Convey the sections in the staining reagents • Stick sections with 2% celloidin solution or stick sections with 1% gelatinous water • Eliminate celloidin with oil of cloves, ethanol–ether solution and 100% ethanol.
Certain chemicals are not chemically fixed on tissues, but they are dissolved into them. These substances are not dyes but lysochromes. They are used to stain lipids. Most dyes are organic synthetic products; a few have a natural origin, essentially vegetal. 2 Chromophores The main chromophore groups are • • • • • Azoic Azine Indamine or thiazine Nitro Kinonic form of aromatic molecules and naphtokinones The higher the number of chromophoric groups, the more intense the coloration will be. 3 Acidic and Basic Dyes In classic histology, it is recognized that acidic dyes are cytoplasmic and basic dyes are nuclear.