By Ülo Langel

Divided into 3 elements this quantity summarizes crucial parts of Cell-Penetrating Peptides (CPP) study . half one in brief offers the ancient historical past of CPP stories and the classifications of the to be had CPPs, after which summarizes the ways for prediction of novel CPPs. half ordinarily describes the tools for experiences of “naked” CPPs, that's, CPPs with no conjugated cargos. final yet no longer least half 3 provides a consultant and short precis of performance problems with CPPs, either in vitro and in vivo. As a quantity within the hugely profitable Methods in Molecular Biology sequence, chapters include introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, effortlessly reproducible protocols, and tips about troubleshooting and keeping off recognized pitfalls.

Concise and easy-to-use, Cell-Penetrating Peptides: tools and Protocols, moment Edition hopes to elevate proper questions for extra improvement.

Show description

Read Online or Download Cell-Penetrating Peptides: Methods and Protocols PDF

Similar molecular biology books

Cell Mechanics

Mobile mechanics is the sector of analysis that appears at how cells observe, regulate, and reply to the actual homes of the mobile setting. Cells speak with one another via chemical and actual indications that are excited by various strategy from embryogenesis and wound therapeutic to pathological stipulations akin to cancerous invasion.

Carotenoids and Retinoids

The presence of carotenoids within the nutrition and their function in human overall healthiness has turn into a topic of remarkable curiosity. The chapters during this e-book characterize an account of the data provided at a contemporary workshop, mixed with numerous extra invited contributions to hide themes extra thoroughly which are at the moment on the innovative of study.

Plant cytogenetics

Cytogenetics performs an enormous function in realizing the chromosomal and genetic structure of plant species. Plant Cytogenetics, 3rd version follows the culture of its predecessors providing theoretical and sensible facets of plant cytogenetics. Chapters describe right dealing with of plant chromosomes, equipment in plant cytogenetics, phone department, replica tools, chromosome nomenclature, karyotype research, chromosomal aberrations, genome research, transgenic vegetation, and cytogenetics in plant breeding.

Extra resources for Cell-Penetrating Peptides: Methods and Protocols

Sample text

J Biol Chem 269: 10444–10450 5. Derossi D, Calvet S, Trembleau A et al (1996) Cell internalization of the third helix of the Antennapedia homeodomain is receptorindependent. J Biol Chem 271: 18188–18193 6. Derossi D, Chassaing G, Prochiantz A (1998) Trojan peptides: the penetratin system for intracellular delivery. Trends Cell Biol 8:84–87 7. Lee SH, Castagner B, Leroux JC (2013) Is there a future for cell-penetrating peptides in oligonucleotide delivery? Eur J Pharm Biopharm 85:5–11 8. Abes R, Arzumanov AA, Moulton HM et al (2007) Cell-penetrating-peptide-based deliv- 9.

Hariton-Gazal E, Feder R, Mor A et al (2002) Targeting of nonkaryophilic cell-permeable peptides into the nuclei of intact cells by covalently attached nuclear localization signals. Biochemistry 41:9208–9214 81. Mano M, Henriques A, Paiva A et al (2006) Cellular uptake of S413-PV peptide occurs upon conformational changes induced by peptide-membrane interactions. Biochim Biophys Acta 1758:336–346 82. Soomets U, Lindgren M, Gallet X et al (2000) Deletion analogues of transportan. Biochim Biophys Acta 1467:165–176 83.

Consequences on the mechanism of cell penetration. Biochim Biophys Acta 1758: 1846–1851 Rydstrom A, Deshayes S, Konate K et al (2011) Direct translocation as major cellular uptake for CADY self-assembling peptidebased nanoparticles. PLoS One 6:e25924 Joliot A, Prochiantz A (2008) Homeoproteins as natural penetratin cargoes with signaling properties. Adv Drug Deliv Rev 60:608–613 Layalle S, Volovitch M, Mugat B et al (2011) Engrailed homeoprotein acts as a signaling molecule in the developing fly.

Download PDF sample

Rated 4.95 of 5 – based on 21 votes