By Hidezo Mori, Hikaru Matsuda
The proven fact that the cardiovascular process transports oxygen and nutrition to all components of the physique makes any possibility to its wellbeing and fitness a major possibility to organs, tissues, and cells. This primary fact underlines the significance of analysis into cardiovascular regeneration and, accordingly, of this assortment outlining the most recent advancements within the box. With enter from some of the top researchers and practitioners, this ebook describes innovations for healing angiogenesis and vasculogenesis for the remedy of ischemic illnesses and examines the present techniques to angiogenic cytokines, cardiovascular stem cells, and tissue-engineering instruments. the newest medical effects utilizing bone marrow-derived mononuclear cellphone remedy for intractable circulatory issues also are lined. This physique of labor may be a helpful source for pros within the fields of cardiovascular surgical procedure, regenerative medication, and tissue engineering.
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Additional info for Cardiovascular regeneration therapies using tissue engineering approaches
2004) Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature 428(6983):664-8 Noishiki Y, Tomizawa Y, Yamane Y, Matsumoto A (1996) Autocrine angiogenic vascular prosthesis with bone marrow transplantation. Nat Med 2(l):90-93 Oh H, Bradfute SB, Gallardo TD, Nakamura T, Gaussin V, Mishina Y, Pocius J, Michael LH, Behringer RR, Garry DJ, Entman ML, Schneider MD. (2003) Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction.
There are no immunological or ethical issues because of the autologous source. Bone marrow comprises two major systems; one haematopoietic and the other mesenchymal. Bone, cartilage, and fat derive from "mesenchymal stem cells". However, the lack of a universal way to identify "mesenchymal stem cells" represents a major obstacle. In 1999, Makino S, et al. (Makino S, et al. 1999) and our group (Tomita S, et al. 1999) reported that cardiomyocytes could be generated from 5azacytidine-treated bone marrow cells.
3). Pulsatile myocardial tissue engineered by layering cell sheets In native heart tissue, cardiomyocytes are tightly interconnected with gap junctions and pulsate simultaneously. Therefore, it is critical whether electrical and morphological communications are established between layered cardiomyocyte sheets. To examine this point, two neonatal rat cardiomyocyte sheets, which were harvested from PIPAAm-grafted dishes, were overlaid. Two electrodes were set at monolayer parts of both cell sheets.