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24 ORGANELLES AND MEMBRANES [1] a d e c r e a s e d ability to sustain flight. 147,1,54As in m a m m a l i a n mitochondria, glycerol-3-phosphate d e h y d r o g e n a s e is stimulated by Ca 2+. 146,155 Massive and rapid m o n o v a l e n t cation p e r m e a t i o n is electrophoretic and requires a potential gradient and p e r m e a n t anions. 145 Calcium ion transport is unclear. An earlier report indicated no high-affinity sites and v e r y few of low affinity. 151 Recently, there has b e e n a d e m o n s t r a t i o n o f an energylinked Ruthenium-red-sensitive Ca 2+ transport with less than one carrier p e r c y t o c h r o m e oxidase.

Yield and Characterization of Mitochondria The characterization and yield of the mitochondria are given in Table I. Most of the mitochondria (80% or more) are isolated from the "nuclear fraction" (mitochondria R 0 , which in other procedures is usually discarded in the preparation of mitochondria from liver. The recovery of mitochondria (both mitochondria R~ and R2) is approximately two-thirds of the amount estimated to be in the liver. 12 Mitochondria R1 are intact and are mainly in the condensed configuration.

10 S e r u m t u b e s m a d e b y N U N C o f D e n m a r k ( d i s t r i b u t e d b y V a n g u a r d I n t e r n a t i o n a l , B o x 321, R e d B a n k , N e w J e r s e y ) a n d s m a l l p o l y p r o p y l e n e b o t t l e s w i t h c a p s ( s u c h as t h o s e d i s t r i b u t e d b y Bel A r t s , P e q u a n n o c k , N e w J e r s e y , c a t a l o g u e N o . 10605) are c o n v e n i e n t for q u i c k - f r e e z i n g a n d s t o r a g e o f s a m p l e s . [4] ISOLATION OF HEART MITOCHONDRIA 39 liquid-nitrogen refrigerator at - 196 ° or in a low-temperature freezer at - 8 0 °.

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