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The significantly acclaimed laboratory common, Methods in Enzymology, is without doubt one of the such a lot hugely revered guides within the box of biochemistry. considering the fact that 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable this day - really a vital booklet for researchers in all fields of lifestyles sciences
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Extra info for Bioluminescence and Chemiluminescence
In aqueous solutions, it is sensitive to extremes in pH, estsecially in the presence of oxygen and light. , 7% per hour at 4° in aqueous pyridine). Alkaline solutions, in the presence of oxygen, can give rise to dehydroluciferin. For these reasons, if crystalline luciferin is to be stored for extended periods of time, it should be well desiccated in light-tight containers and under nitrogen atmosphere. Under these conditions luciferin can be stored indefinitely without oxidation, racemization, or photodecomposition.
RASMUSSEN The purification of extracts of firefly tails for use in the analysis of ATP requires methods that can be applied to rather small amounts of material. The fractionation requirements are, however, well defined: (1) removal of ATP and other substances of low molecular weight to lower the blank values, and (2) removal of interfering enzymes to decrease the systematic errors of the analysis. ' Luciferin and dehydro' R. Nielsen and H. N. R a s m u s s e n , Acta Chem. Scand. 22, 1757 (1968).
J. Biochem. 67, 1 (1976). inhibition. 5 to 250 pM ATP. The rapid kinetics of ATP synthesis initiated by a pulse of oxygen to reduced, anaerobic inner membrane vesicles is shown in Fig. 8A. One syringe of a stopped-flow apparatus contains anaerobic vesicles and ADP. and the other syringe contains an oxygenated solution of luciferin and luciferase. The contents of the two syringes are rapidly mixed, and the resulting luminescence signal increases in a linear fashion. A lag time of 40-60 msec precedes the development of any luminescence.