By Nathan P. Kaplan, William B. Jakoby, Meir Wilchek

The seriously acclaimed laboratory usual, tools in Enzymology, is among the such a lot hugely revered courses within the box of biochemistry. seeing that 1955, each one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - really a necessary book for researchers in all fields of existence sciences.

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Chem. 241, 4295 (1966). lJ M. Brockhaus and J. Lehmann, FEBS Lett. 62, 154 (1976). 'ell. Chaimovich, R. J. Vaughan, and F. H. Westheimer, J. Am. Chem. Soe. 90, 4088 (1968) ; J. Sharer, P. Baronowsky, R. Lawsen, F. Finn, and F. H. Westheimer, J. Biol. Chem. 241, 421 (1966). [3] 37 MECHANISM-BASED IRREVERSIBLE INHIBITORS These compounds are thought to function by undergoing elimination reaction with certain enzymes to give rise to reactive Michael acceptors. ~'\ H (7) "~N~'\ H fl-Chloroalanine has been found to be an irreversible inhibitor of the pyridoxal phosphate-linked fl-aspartate decarboxylase, 1~ aspartate aminotransferase, '8 and alanine racemase.

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A. L. Fluharty and C. E. Ballou, J. Biol. Chem. 234, 2517 (1958). ~R dehydrogenase ] H~,.. e. o. o- -ope| hh (CH2)2 NH~--C--COOH H a j. Everse, E. C. Zoll, L. Kahan, and N. O. Kaplan, Bioorg. Chem. 1, 207 (1971). b W. B. Noroa, A. D. Winer, A. J. Glaid, and G. W. Schwert, J. Biol. Chem. 234, 1143 (1959). c E. Racker, V. Klybas, and M. Schramm, J. Biol. Chem. 234, 2510 (1958). d A. L. Fluharty and C. E. Ballou, J. Biol. Chem. 234, 2517 (1958). • D. Northrop and H. G. Wood, J. Biol. Chem. 244, 5810 (1969).

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